Proteomics and Spectroscopy

USC CIRM ASCEND Shared Resource Laboratory (SRL)

The CIRM ASCEND (Advancing Stem Cell Education and Novel Discoveries) Shared Resource Laboratory (SRL) provides expertise in stem cell and organoid models, coupled with state-of-the-art single cell and spatial sequencing technologies, and linked training courses to the broad California research community. It welcomes students and collaborators from the higher education systems, high school students, and all areas of biotechnology. The CIRM ASCEND SRL is part of a network of SRLs funded by CIRM that are aimed to democratize science by making challenging models, experiments, and tools accessible. For a full set of services provided in California, visit the CIRM website at https://www.cirm.ca.gov.

Eli and Edythe Broad CIRM Center (BCC) for Regenerative Medicine and Stem Cell Research

Keck School of Medicine University of Southern California
1975 Zonal Ave, Los Angeles, CA 90033

Website

Dornsife PLASMA Center

Plasma Laboratory for Atmosphere, Solid Earth, and Marine Analyses
Equipment and services: Neptune MC-ICPMS, Agilent 8900 QQQ-ICPMS, Agilent 5110 ICP-OES, Mercury Analyzer NIC MA-3000,

ZHS 320

kayoung@usc.edu

Center for Peptide and Protein Engineering

The USC Center for Peptide and Protein Engineering (CPPE) aims to put USC in a position of undisputed leadership in peptide and protein science by creating functional peptides and proteins as imaging agents, diagnostics and therapies. This core laboratory is housed in the Michelson Center for Convergent Biosciences and serves the USC Community to generate new peptide and protein tools. New technology advances in several fields now provide the foundation for high-throughput molecular engineering—creating new peptides and antibody-like proteins that bind specific targets on a genomic scale.

Equipment and services include: A la carte and comprehensive peptide synthesis and purification services for the USC and general community. End-to-end production of peptides includes: assistance in peptide design, Fmoc-based solid-phase peptide synthesis, peptide purification, conformation of peptide identity by mass spectrometry, and assessment of purity by reverse-phase HPLC. Peptide synthesis is performed on a Biotage Syro I Multi-Peptide Synthesizer (supported by a grant from the USC Core Instrumentation Fund) at 50 μmol scale up to 20 amino acids. Peptide purification is performed via Biotage reverse-phase flash chromatography and/or reverse-phase HPLC chromatography using Agilent HPLC and LCMS equipment.

2nd floor
USC Michelson Center for Convergent Bioscience
MCB 267

Website

Nanobiophysics Core

The Center of Excellence in NanoBiophysics supports faculty and students across the university in the study of molecular nano-assembly, structures and functions. Housed in the Dornsife College, the Center is used by research programs in structural biology, nanotechnology, anomedicine, molecular biology, biochemistry, and materials sciences.

3616 Trousdale Parkway
AHF B15
Los Angeles, CA 90089

Website

Mass Spectrometry Core

The Mass Spectrometry Core at USC Mann School of Pharmacy serves the scientific research community at USC as well as external collaborators. Our Core is equipped with state-of-the-art instruments for imaging mass spectrometry (IMS), high-throughput screening, and quantitative metabolomic and proteomics. Our Core is dedicated to actively collaborate with internal and external research groups in providing comprehensive service in MALDI imaging and high-throughput screening of small molecules, peptides, and proteins using mass spectrometry.

University of Southern California
1985 Zonal Avenue
Los Angeles, CA 90089-9121
Website

Spatial Biology and Genomics Core

Located at the CHLA Saban Research Institute, the Proteomics Facility utilizes proteomics with advanced mass spectrometry to investigate protein functions and roles in disease. Equipment & services include: proteomics and mass spectrometry services utilizing Eksigent nanoLC-2D and Thermo LTQ Orbitrap XL. Proteomics software includes Thermo Bioworks and Protein Scaffold Software.

Children’s Hospital Los Angeles, CHLA
Smith Research Tower
4546 Sunset Blvd., Rm 106, MS#64
Los Angeles, CA 90027

Spatial Biology and Genomics Core | Children’s Hospital Los Angeles