The USC Center for Peptide and Protein Engineering (CPPE) aims to put USC in a position of undisputed leadership in peptide and protein science by creating functional peptides and proteins as imaging agents, diagnostics and therapies. This core laboratory is housed in the Michelson Center for Convergent Biosciences and serves the USC Community to generate new peptide and protein tools. New technology advances in several fields now provide the foundation for high-throughput molecular engineering—creating new peptides and antibody-like proteins that bind specific targets on a genomic scale.
Equipment and services include: A la carte and comprehensive peptide synthesis and purification services for the USC and general community. End-to-end production of peptides includes: assistance in peptide design, Fmoc-based solid-phase peptide synthesis, peptide purification, conformation of peptide identity by mass spectrometry, and assessment of purity by reverse-phase HPLC. Peptide synthesis is performed on a Biotage Syro I Multi-Peptide Synthesizer (supported by a grant from the USC Core Instrumentation Fund) at 50 μmol scale up to 20 amino acids. Peptide purification is performed via Biotage reverse-phase flash chromatography and/or reverse-phase HPLC chromatography using Agilent HPLC and LCMS equipment.
USC Michelson Center for Convergent Bioscience