Research Initiatives and Infrastructure

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Children's Hospital Los Angeles

Molecular Genomics/Microarray

The USC Norris Comprehensive Cancer Center Microarray Core is located at the CHLA pediatric facility. Services include: gene expression profiling with a variety of arrays, including human, mouse and rat; mutation analyses (p53); HIV, yeast, and bacterial genome analyses; and SNP analyses. RNA or DNA is extracted, purified, labeled, and in the case of expression chips, converted into double stranded cDNA, prior to cRNA, labeling and fragmentation.

Children’s Hospital Los Angeles, CHLA
4650 Sunset Blvd, MS#103
Los Angeles, CA, 90027
Website

Good Manufacturing Practice Core

The Good Manufacturing Practice (GMP) Core, is located at the Saban Research Institute at CHLA. Services include: the manufacture of therapeutic grade cell-based products under GMP conditions for use in clinical trials.

Children’s Hospital Los Angeles, CHLA
Smith Research Tower
4546 Sunset Blvd., Rm 106, MS#64
Los Angeles, CA 90027
Website

Small Animal Imaging Core

The Research Imaging Core at the Saban Research Institute, CHLA, is a full-service in vivo imaging laboratory providing non-invasive capabilities for use on mammalian models. Equipment & services include: data collection, processing and interpretation and access to core instrumentation: a Xenogen for optical imaging (bioluminescence and fluorescence), a 7T micro Magnetic Resonance Imager (MRI), a micro X-ray Computed Tomography (CT) instrument and a Gamma Medica combined CT micro Single Photon Emission Computed Tomography (SPECT).

Children’s Hospital Los Angeles, CHLA
Saban Institute Small Animal Imaging Research Center
4661 Sunset Blvd.
Los Angeles, CA 90027

Small Animal Imaging Core | Children’s Hospital Los Angeles

Spatial Biology and Genomics Core

The Spatial Biology and Genomics (SBG) Core at CHLA provides our research community with new, rapidly evolving technologies and instrumentation options for projects of any scale – individual researchers to large international teams. Core services include spatial proteomics on the Akoya Pheno-Cycler Fusion platform, spatial transcriptomics on the GenePS system from Spatial Genomics, and the full array of single cell genomics options from 10X Genomics. Other services include Microbiome Metagenomics (16S and Whole Genome), RNA expression profiling and high throughput RNA/ DNA Next Generation Sequencing with advanced data analysis. With a focus on rapid, accurate, reproducible high-throughput services and the bioinformatics support to interpret the results, the SBG Core is committed to rigorous quality control standards to support the use of biological sciences for the advancement of biomedical research.

Children’s Hospital Los Angeles, CHLA
Smith Research Tower
4650 Sunset Blvd., SRT 911, MS#64
Los Angeles, CA 90027

Spatial Biology and Genomics Core | Children’s Hospital Los Angeles

Cellular Imaging Core

The Cellular Imaging Core houses light microscopy and digital image processing equipment to support cell biology research. 

Services include: consultation on the design, execution, and analysis of fixed and live cell imaging experiments as well as training in the utilization of Core facilities.

Equipment includes: Lightsheet microscope (LaVision Biotec Ultramicroscope II), laser scanning confocal microscopes (Zeiss LSM 710 & 700), live cell imaging microscope and spinning disk confocal (BD/CrEST CARVII) with temperature/CO2/O2 control, widefield fluorescence microscopes (Leica DMI6000B AFC & Nikon Eclipse Ti-E), Optical Projection Tomography (OPT) microscope, 3D image rendering and analysis workstation with Arivis Vision4D and AutoQuant deconvolution software, 2D image analysis workstation with MetaMorph software.

Children’s Hospital Los Angeles, CHLA
Smith Research Tower
4546 Sunset Blvd
Los Angeles, CA 90027
Website