Children's Hospital Los Angeles

Vector Core

The Vector Core provides researchers with state of the art technology, facilities, support staff and services and a broad range of resources. Services include: assistance in the design and construction viral vectors and viral particles for gene-modified in vitro/in vivo studies. The core also provides consulting services to identify researcher gene transfer needs, determine the best way to produce gene-modified cells, and to assist in experimental troubleshooting. Equipment includes: Hollow fiber tangential flow filtration (TFF) columns (Spectrum Labs) to filter and concentrate lentiviral vectors up to 100 fold with high recovery (>90%).

Children’s Hospital Los Angles, CHLA
Smith Research Tower
4546 Sunset Blvd., Rm 405
Los Angeles, CA 90027

Molecular Genomics/Microarray

The USC Norris Comprehensive Cancer Center Microarray Core is located at the CHLA pediatric facility. Services include: gene expression profiling with a variety of arrays, including human, mouse and rat; mutation analyses (p53); HIV, yeast, and bacterial genome analyses; and SNP analyses. RNA or DNA is extracted, purified, labeled, and in the case of expression chips, converted into double stranded cDNA, prior to cRNA, labeling and fragmentation.

Children’s Hospital Los Angeles, CHLA
4650 Sunset Blvd, MS#103
Los Angeles, CA, 90027
Website

Good Manufacturing Practice Core

The Good Manufacturing Practice (GMP) Core, is located at the Saban Research Institute at CHLA. Services include: the manufacture of therapeutic grade cell-based products under GMP conditions for use in clinical trials.

Children’s Hospital Los Angeles, CHLA
Smith Research Tower
4546 Sunset Blvd., Rm 106, MS#64
Los Angeles, CA 90027
Website

Gene Targeting & Pathology Core

The Genome Targeting and Pathology Core provides genetically manipulated mice for biomedical studies. Services include: conditional /tissue-specific knockouts, knockins & lentiviral transgenesis, tissue paraffin embedding and sectioning, routine H&E staining and genotyping of tail DNA.

Children’s Hospital Los Angeles
4546 Sunset Blvd
Los Angeles, CA 90027

Research Imaging Core at the Saban Research Institute

The Research Imaging Core at the Saban Research Institute, CHLA, is a full-service in vivo imaging laboratory providing non-invasive capabilities for use on mammalian models. Equipment & services include: data collection, processing and interpretation and access to core instrumentation: a Xenogen for optical imaging (bioluminescence and fluorescence), a 7T micro Magnetic Resonance Imager (MRI), a micro X-ray Computed Tomography (CT) instrument and a Gamma Medica combined CT micro Single Photon Emission Computed Tomography (SPECT).

Children’s Hospital Los Angeles, CHLA
Saban Institute Small Animal Imaging Research Center
4661 Sunset Blvd.
Los Angeles, CA 90027

Proteomics Core

Located at the CHLA Saban Research Institute, the Proteomics Facility utilizes proteomics with advanced mass spectrometry to investigate protein functions and roles in disease. Equipment & services include: poteomics and mass spectrometry services utilizing Eksigent nanoLC-2D and Thermo LTQ Orbitrap XL. Proteomics software includes Thermo Bioworks and Protein Scaffold Software.

Children’s Hospital Los Angeles, CHLA
Smith Research Tower
4546 Sunset Blvd., Rm 106, MS#64
Los Angeles, CA 90027