The USC Mann School of Pharmacy Lentiviral Laboratory provides services for production of small-scale lentiviral stocks for delivery and expression of transgenes or shRNA of interest. The engineered lentiviral constructs are either provided by the end-users or produced in the lab according to the needs of the customers. We also help in stable cell line generation and characterization.
Gene Expression Profiling, Genotyping, and Genomic Analysis
Vector Core
The Vector Core provides researchers with state of the art technology, facilities, support staff and services and a broad range of resources. Services include: assistance in the design and construction viral vectors and viral particles for gene-modified in vitro/in vivo studies. The core also provides consulting services to identify researcher gene transfer needs, determine the best way to produce gene-modified cells, and to assist in experimental troubleshooting. Equipment includes: Hollow fiber tangential flow filtration (TFF) columns (Spectrum Labs) to filter and concentrate lentiviral vectors up to 100 fold with high recovery (>90%).
Children’s Hospital Los Angles, CHLA
Smith Research Tower
4546 Sunset Blvd., Rm 405
Los Angeles, CA 90027
Molecular Genomics/Microarray
The USC Norris Comprehensive Cancer Center Microarray Core is located at the CHLA pediatric facility. Services include: gene expression profiling with a variety of arrays, including human, mouse and rat; mutation analyses (p53); HIV, yeast, and bacterial genome analyses; and SNP analyses. RNA or DNA is extracted, purified, labeled, and in the case of expression chips, converted into double stranded cDNA, prior to cRNA, labeling and fragmentation.
Children’s Hospital Los Angeles, CHLA
4650 Sunset Blvd, MS#103
Los Angeles, CA, 90027
Website
Gene Targeting & Pathology Core
The Genome Targeting and Pathology Core provides genetically manipulated mice for biomedical studies. Services include: conditional /tissue-specific knockouts, knockins & lentiviral transgenesis, tissue paraffin embedding and sectioning, routine H&E staining and genotyping of tail DNA.
Children’s Hospital Los Angeles
4546 Sunset Blvd
Los Angeles, CA 90027
Choi Family Therapeutic Screening Facility
The Choi Family Therapeutic Screening Facility enables investigators from USC and beyond to improve their understanding of diseases and accelerate the discovery of potential therapeutic drugs.
Equipment and services include: extensive chemical libraries, state-of-the-art screening equipment and trained technicians.
Health Sciences Campus
Eli and Edythe Broad CIRM Center
1425 San Pablo Street, BCC 205A
Los Angeles, CA 90033
Website