Cell Culture Core is a recharge facility that supports a wide range of research needs in Cell Culture. Our areas of expertise include baculovirus expression of G-Protein Coupled Receptors (GPCRs) in SF9 insect cells, mammalian expression of GPCRs in HEK- 293F suspension cells, plating of adherent cells for imaging, cell maintenance, banking, mycoplasma testing, cell culture lab set up, and consulting. Our work has supported crystallization and structure determination of more than thirty unique human G protein-coupled receptor structures, as well as provided material for collaborative biophysical and pharmacology studies in the labs of numerous collaborators. In addition to protein expression, the Cell Culture Core has aided research in antibody expression, cell imaging, and functional assays.
Equipment and services include: State-of-the-art equipment and materials for any BSL2 level insect and mammalian cell lines, both in suspension and adherent platforms. Multiple biosafety cabinets, including an independent workstation complete with its own cell counter, shaker, and incubator. Further on-site equipment includes several CO2 Incubators/shakers including large capacity Multitron Pro Shakers, and Cytek (previously Millipore/Guava®) easyCyte Flow Cytometers to measure cell markers and protein expression efficiency.
1002 Childs Way
MCB 360
Los Angeles, CA 90089
https://dornsife.usc.edu/bridgecellculture/